The interaction between the analytes and the chemoreceptor layers was determined by reflectometric interference spectroscopy rifs. The shift of the interference pattern caused by a change of the optical thickness of a sensitive layer, due to the influence of analyte, is investigated. Rifs abbreviation stands for reflectometric interference spectroscopy. Reflectometric interference spectroscopy springerlink. Read interaction between volatile organic compounds and cyclopeptides detected with reflectometric interference spectroscopy, analytica chimica acta on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Quantification of quaternary mixtures of low alcohols in. Related content enhancing chemical identification efficiency by saw sensor transients through a data enrichment and. Fast transient cytokinereceptor interactions monitored in real time by reflectometric interference spectroscopy jacob piehler1 and gideon schreiber department of biological chemistry, the weizmann institute of science, 76100 rehovot, israel. The conventional rifs technique is an appropriate method to detect interfacial interactions at the solidgas or solidliquid interface in the case of thin films with a thickness of a few hundred nanometers, but when a significantly lower layer thickness.
Fast transient cytokinereceptor interactions monitored in. Reflectometric interference spectroscopy rifs is a physical method based on the interference of white light at thin films, which is used to investigate molecular interaction. A setup of the sensor having dual light emitting diodes leds and one photo detector are introduced. Exploration of interactions between membrane proteins. Figure 1 shows the schematic diagram of a typical reflectometric setup with a polarizer and calculation of the most notable parameters. Ir absorption and reflectometric interference spectroscopy.
B thin film stack, with a glass slide thickness of 1 mm and a refractive index of 1. Sensing of volatile organic compounds using a simplified reflectometric interference spectroscopy setup d. A standard protocol for a binding inhibition assay is given. Detection of whole cells using reflectometric interference. A microfluidic reflectometric interference spectroscopy rifsbased sensor was fabricated to investigate the activity of multidrug resistanceassociated protein 1 mrp1, applied as a model membrane protein. The conception of the polarization reflectometric interference spectroscopy prifs method is similar to the conventional rifs technique, the difference lies only in the implementation of the measurement. The recognition properties of the nanosensors were evaluated by reflectometric interferometric spectroscopy. We present a simplified optical sensor system using the principle of reflectometric interference spectroscopy rifs for monitoring organic solvent vapors in air. Preparation of silicon nitride biochips for reflectometric. Ftir reflection spectra of the layers were recorded showing the characteristic ir absorption bands of the polymer and the interference pattern generated by. It was found that the affinity of the analytes for the cyclopeptides was higher than for the linear peptide. Along with these setups, there are several other interferometric configurations, such as reflectometric interference spectroscopy rifs 203, spectralphase interferometry spi 204 or spectralcorrelation interferometry sci, among others. Reflectometric interference spectroscopy rifs as a new.
Detection of whole cells using reflectometric interference spectroscopy article in physica status solidi a 2095. Development of a polarimetric based optical biosensor. Sarcosine was recently identified as a differential metabolite that is present in urine in elevated concentration as prostate cancer develops metastases. Introduction of carboxy groups on a silicon nitride sensor chip to immobilize antiafp monoclonal. Combined reflectometric interference spectroscopy and quartz crystal microbalance detect differential adsorption of lipid vesicles with different phase transition temperatures on sio 2, tio 2, and au surfaces taisuke kojima, department of biomedical engineering, georgia institute of technology, 950 atlantic drive.
The interference pattern is detected by only four wavelengths, in contrast to. By adapting reflectometric interference spectroscopy for simultaneous reading of the whole transducer plate we were able to detect the binding of thrombin in all the wells of the microplates online, in parallel, and time resolved. It overcomes principal problems of any direct optical detection technique. Interference by anions that form low volatility complexes with the analyte, and thus reduce the atoms formed. Reflectometric optical sensors are based on the method called reflectometric interference spectroscopy rifs where interference spectroscopy is used to record the reflectance spectra at thin transparent films 36. The aim of this study is to prepare reflectometric interference spectroscopy rifs nanosensors for the detection of sarcosine. Reflectometric interference spectroscopy rifs is a labelfree, timeresolved technique for detecting interactions of molecules immobilized on a surface with ligands in solution. Reflectometric interference spectroscopy rifs is based on the multiple reflectance of white light at a layer system. Labelfree detection of glycoproteins using reflectometric. A reflectometric interferometric nanosensor for sarcosine. The potential of a labelfree detection method, reflectometric interference spectroscopy rifs, for temperaturedependent dna hybridisation experiments for example in single nucleotide. The glass frit nebulizer produces a much finer aerosol than the conventional pneumatic nebulizer. Reflectometric interference spectroscopy request pdf.
Some interference effects may also be related to the process of droplet size discrimination within the spray chamber. Reflectometric interference spectroscopy rifs as a new tool to measure in the complex matrix milk at low analyte concentration. Spectroscopic ellipsometry was used to investigate the physical thickness of the peptide layer. Interaction between volatile organic compounds and. When ovalbumin ova, a model glycoprotein, was injected, the sensor response increased due to. Here we show that rifs also permits the detection of the adhesion of tissue culture cells to a functionalized surface in a flow system. Thin film thickness variation measurement using dual leds. Biomimetic assembly of polyelectrolyte multilayers. Reflectometric interference spectroscopybased sensing for. Analytical features of an optical biosensor based on. Rifs stands for reflectometric interference spectroscopy. The parts of the light reflected at different interfaces of the layer system superimpose and form an interference pattern.
Media in category reflectometric interference spectroscopy the following 6 files are in this category, out of 6 total. In this paper, we present an improved reflectometric interference spectroscopy rifs sensor principle which is suitable for thin films. View enhanced pdf access article on wiley online library html view. Fabrication of carboxylated silicon nitride sensor chips. Vesicles containing mrp1 were immobilized simply by injecting a. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy rifs. Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance spr. Combined reflectometric interference spectroscopy and.
Due to its high accuracy and resolution, the rifs technique has primarily been utilized for biomolecular interaction analysis bia. The rifs technique is based on the spectral shift of the interference pattern of fabryperot fringes. A model to estimate thickness variation is built based on rifs. Reflectometric interference spectroscopy listed as rifs. What is the abbreviation for reflectometric interference spectroscopy. Reflectometric interference spectroscopy wikipedia. Read reflectometric interference spectroscopy based sensing for evaluating biodegradability of polymeric thin films, acta biomaterialia on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Rifs detects the adsorbed mass based on the reflectance and predicts the adsorbed condition by modeling the reflection spectra using the transfer matrix method. Pdf suitability of hyperbranched polyester for sensoric. Can be corrected by releasing agents cations added to preferentially react protecting agents e. Moreover, some configurations can be found in commercial devices.
Development of an assay for labelfree highthroughput. Antifungal susceptibility testing of aspergillus niger on. Suitability of hyperbranched polyester for sensoric applications investigation with reflectometric interference spectroscopy. Chiral discrimination using piezoelectric and optical gas. In this study, we report labelfree detection of alphafetoprotein afp, which has been used as a biomarker for hepatocellular carcinoma, by a microfluidic reflectometric interference spectroscopy rifs system adopting a simple halogen light source and an inexpensive siliconbased sensor chip. Sensing of volatile organic compounds using a simplified. Reflectometric interference spectroscopy free download as pdf file. The conventional rifs technique is an appropriate method to detect interfacial interactions at the solidgas or solidliquid interface in the case of thin films with a thickness of a few hundred nanometers, but when a significantly lower layer thickness 100. The potential of thin film thickness variation measurement method, reflectometric interference spectroscopy rifs, for a compact labelfree biosensor is investigated. The theoretical concept of polarization reflectometric. This definition appears somewhat frequently and is found in the following acronym finder categories. Reflectometric interference spectroscopy was applied to characterize changes in the optical thickness. Preparation of silicon nitride biochips for reflectometric interference spectroscopic rifs analysis of.
Here, a combined reflectometric interference spectroscopy rifs and qcm is developed to simultaneously analyze adsorption of biomolecules. Reflectometric interference spectroscopy scientific. Overview reflectometric interference spectroscopy rifs theory, setup and applications irrifs setup results obtained with the new irrifs setup. It is not as susceptible to clogging as a conventional nebulizer and.
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